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westar supernova ecl reagent  (Cyanagen)


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    Cyanagen westar supernova ecl reagent
    Westar Supernova Ecl Reagent, supplied by Cyanagen, used in various techniques. Bioz Stars score: 96/100, based on 328 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ecl+westar+supernova/WESTAR+SUPERNOVA/bio_rxiv__64898__2026__03__29__715100-246-7-11
    Average 96 stars, based on 328 article reviews
    westar supernova ecl reagent - by Bioz Stars, 2026-09
    96/100 stars

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    Article Title: Comparative Effects of Dexamethasone and ASC Secretome in an Ex Vivo Osteoarthritis Co-Culture Model
    Article Snippet: Specific signals were detected using appropriate horseradish peroxidase-conjugated secondary antibodies (Thermo Fisher Scientific, Waltham, MA, USA; 1:3000 dilution) and visualized with ECL Westar Supernova (Cyanagen, Bologna, Italy).

    Article Title: Insights into mesenchymal stem/stromal cell conditioned media as a long-lasting treatment for pain and psychiatric comorbidities in a murine model of osteoarthritis.
    Article Snippet: Specific signals were detected using appropriate horseradish peroxidase-conjugated secondary antibodies and visualized with ECL Westar Supernova (Cyanagen, Bologna, Italy).

    Article Title: Comparative Effects of Dexamethasone and ASC Secretome in an Ex Vivo Osteoarthritis Co-Culture Model
    Article Snippet: Specific signals were detected using appropriate horseradish peroxidase-conjugated secondary antibodies (Thermo Fisher Scientific, Waltham, MA, USA; 1:3000 dilution) and visualized with ECL Westar Supernova (Cyanagen, Bologna, Italy).

    Article Title: Evaluation of MSC‐Secretome Effects in an Ex Vivo Compartmentalized Osteochondral Interface Model
    Article Snippet: Specific signals were detected using appropriate horseradish peroxidase‐conjugated secondary antibodies (Thermo Fisher Scientific, Waltham, MA, USA; 1:3000 dilution) and visualized with ECL Westar Supernova (Cyanagen, Bologna, IT).

    Incubation:

    Article Title: Connexin 43 Modulation in Human Chondrocytes, Osteoblasts and Cartilage Explants: Implications for Inflammatory Joint Disorders
    Article Snippet: Primary antibodies raised against Connexin 43 C-terminal (rabbit anti-Connexin43; #3512, Cell Signaling, Danvers, MA, USA, dilution ratio 1:1000) and β-actin (mouse anti-β-actin A2228, Merck, Darmstadt, Germany, dilution ratio 1:5000) were incubated at 4° overnight and at room temperature for 1 h, respectively. .. Specific signals were revealed after the incubation with the appropriate secondary antibodies conjugated to horseradish peroxidase followed by detection with ECL Westar Supernova (Cyanagen, Bologna, Italy). .. Signals were acquired using a Chemidoc Imaging System (Bio-Rad, Milan, Italy) and densitometry was quantified through Image Lab Software version 6.1 (Bio-Rad, Milan, Italy).

    Article Title: Serum starvation affects mitochondrial metabolism of adipose-derived stem/stromal cells.
    Article Snippet: The mouse antiSOD2 primary antibody (sc-137254, 1:500 diluted; Santa Cruz Biotechnology, CA, USA) and the mouse anti-DPR1 (611113, 1:1000 diluted; BD Biosciences, Franklin Lakes, NJ, USA) were incubated overnight at 4°C while the mouse anti-b-Actin primary antibody (A2228, Merck, Darmstadt, Germany, 1:5000 diluted) was incubated for 2 h at room temperature. .. Specific bands were revealed after the incubation with the appropriate secondary antibody conjugated to horseradish peroxidase (goat anti-mouse IgG, 62-6520, 1:6000 diluted; Thermo Fisher Scientific) followed by detection with ECL Westar Supernova (Cyanagen, Bologna, Italy). .. Signals were acquired by Chemidoc Imaging System and densitometry was quantified through Image Lab Software (Bio-Rad).

    Article Title: Connexin 43 Modulation in Human Chondrocytes, Osteoblasts and Cartilage Explants: Implications for Inflammatory Joint Disorders.
    Article Snippet: .. Specific signals were revealed after the incubation with the appropriate secondary antibodies conjugated to horseradish peroxidase followed by detection with ECL Westar Supernova (Cyanagen, Bologna, Italy). .. Signals were acquired using a Chemidoc Imaging System (Bio-Rad, Milan, Italy) and densitometry was quantified through Image Lab Software version 6.1 (Bio-Rad, Milan, Italy).

    Article Title: Lipidomics of Cell Secretome Combined with the Study of Selected Bioactive Lipids in an In Vitro Model of Osteoarthritis
    Article Snippet: For each sample, 10 μg of protein extract were loaded and probed with the primary antibodies rabbit anti-COX2 (Cell Signaling, Danvers, MA, USA, 1:1000 diluted) and goat anti-GAPDH (Santa Cruz Biotechnology, 0.1 μg/μL, 1:1000 diluted). .. Specific bands were revealed upon incubation with appropriate secondary antibodies conjugated to horseradish peroxidase (Rabbit IgG Secondary antibody, Thermo Fisher Scientific, Waltham, MA, USA, dilution 1:10 000; Goat IgG Secondary Antibody, Santa Cruz Biotechnology, CA, USA; 0.1 μg/μL, 1:6000 diluted) followed by detection with ECL Westar Supernova (Cyanagen, Bologna, Italy). .. After image acquisition with ChemiDoc Imaging System, protein expression was quantified through Image Lab Software (Bio-Rad, Milan, Italy).



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